Journal: Cell Proliferation

Article Title: AMOT 130 linking F‐actin to YAP is involved in intervertebral disc degeneration

doi: 10.1111/cpr.12492

Figure Lengend Snippet: Primary antibodies

Article Snippet: Labels were quantified using the ImageJ software. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Antibody Company (catalog number) LATS1 Abcam (ab70562) LATS2 Antibodies‐online (ABIN753568) YAP Santa Cruz (sc‐376830) Phosphorylated YAP (Ser 127) Abcam (ab172374) CTGF Abcam (ab6992) F‐actin Abcam (ab205) AMOT Santa Cruz (sc‐166924) GAPDH Bioss (bs‐2188R) Open in a separate window Primary antibodies

Techniques:

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Inhibition of matrix metalloproteinase expression by selective clearing of senescent dermal fibroblasts attenuates ultraviolet-induced photoaging.

doi: 10.1016/j.biopha.2022.113034

Figure Lengend Snippet: Fig. 2. Selective elimination of senescent cells in UV-irradiated mice skin after treatment with ABT-263 or ABT-737. Hairless mice were exposed to chronic UV irradiation for eight weeks with 100 mJ/cm2 for the first 2 weeks, then 50 mJ/cm2 weekly, then 300 mJ/cm2. Each drug (ABT-263, 5 μM; ABT-737, 10 μM) or vehicle (DMSO) was diluted in PBS (30 μl) and injected intradermally into the dorsal skin for a total of ten doses. (A) Representative images of SA-β-gal staining in mice skin. SA-β-gal (blue color) was detected in 6 µm frozen skin sections from unirradiated control mice injected with vehicle (n = 7, white bars), UVB-irradiated mice injected with vehicle (n = 4, black bars), UVB-irradiated mice injected with ABT-263 (n = 6, blue bars) or ABT-737 (n = 7, red bars). Arrowheads indicate stained cells. (B) Quantitative analysis of the percentage of SA-β-gal positive cells in the indicated mice skin (n = 3–4 mice per group) (C) Western blots and quantification of p21 and p16 protein levels normalized to GAPDH using protein extracts from the skin. (D) p16 and p21 mRNA levels in indicated mice skin, standardized to GAPDH. (E) Representative images of immunofluorescence staining for p16 (red) and DAPI (blue) in the skin of the indicated mice. Right: Quan tification of the percentage of p16-positive cells among DAPI-positive cells in over n = 4 mice per group. Arrowheads indicate stained cells. Data represent means ± SEM, *p < 0.05, * *p < 0.005, * **p < 0.001. p-values were calculated by Student’s t-test. A, Bar = 100 µm; E, Bar = 50 µm.

Article Snippet: The antibodies used included p16 (MA5–17142, Thermo Fisher Scientific; ab189034, Abcam), p21 (556431, BD Pharmingen; ab109199, Abcam), GAPDH (CSB-PA00029A0Rb, Cusabio, Houston, TX, USA), β-actin (CSBPA000350, Cusabio), MMP-1 (Lab Frontier, Seoul, Korea), and type I procollagen (SP1.

Techniques: Irradiation, Injection, Staining, Control, Western Blot, Immunofluorescence

Journal: Oncology Letters

Article Title: Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene

doi: 10.3892/ol.2017.6248

Figure Lengend Snippet: Treatment with EGCG leads to increased p16 mRNA expression. (A) Results of the agarose gel electrophoresis. (B) Quantitated p16 mRNA expression levels relative to GAPDH. **P<0.01 vs. the untreated cells. EGCG, epigallocatechin-3-gallate; p16, cyclin-dependent kinase inhibitor 2A.

Article Snippet: GAPDH was blotted as an internal reference using the anti-GAPDH rabbit polyclonal antibody (cat. no. AB21612-1; 1:5,000; AbSci, LLC, Portland, OR, USA).

Techniques: Expressing, Agarose Gel Electrophoresis

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: The sequences of primers used to amplify MDR1, BSG, BIRC5 and GAPDH mRNAs [3] , [17] .

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Sequencing

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: The protein expressions of P-gp and CD147 in drug-resistant K562/ADR and drug-free K562/ADR leukemic cell lines . Proteins were extracted drug-resistant K562/ADR and drug-free K562/ADR cell lines and separated by 12% SDS-PAGE and transferred onto PVDF membrane. The membranes were probed with anti-CD147, anti-P-gp and detected by enhanced chemiluminescence. (A) Results are representative of three independent experiments by Western blotting and (B) Quantification of P-gp and CD147 expressions in drug-resistant K562/ADR and drug-free K562/ADR cells; the protein levels were normalized to the loading control GAPDH. The ratio of P-gp and CD147 were calculated for each group. Data are presented as mean±SEM of three independent experiments; ⁎ p <0.05 when compared to drug-free K562/ADR cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: SDS Page, Membrane, Western Blot, Control

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: The level of MDR1 and BSG mRNA in drug-resistant K562/ADR and drug-free K562/ADR leukemic cell lines. Total RNA was extracted from cells and the reverse transcription reaction were performed using a RevertAid First Strand cDNA Synthesis kit (Thermo Scientific™). The newly synthesized cDNA was amplified by PCR using specific primers. The PCR product were electrophoresed on 1.2% agarose gel and visualized by ethidium bromide staining. (A) Results are representative of three independent experiments by semiquantitative RT-PCR analysis and (B) quantification of MDR1 (multidrug resistance 1) and BSG (Basigin) mRNA expression in drug-resistant K562/ADR and drug-free K562/ADR cells; the mRNA levels were normalized to the loading control GAPDH. The ratio of MDR1 and BSG mRNAs were calculated for each group. Data are presented as mean ±SEM of three independent experiments; ⁎ p <0.05 when compared to drug-free K562/ADR cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Reverse Transcription, cDNA Synthesis, Synthesized, Amplification, Agarose Gel Electrophoresis, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Control

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: Effect of MEM-M6/6 anti-CD147 on P-gp (A) and CD147 (B) protein expression in drug-resistant K562/ADR cell line. The protein levels after treating drug-resistant K562/ADR cell with (1) 0 μg/mL MEM-M6/6, (2) 0.625 μg/mL, (3) 1.25 μg/mL, (4) 2.5 μg/mL, and (5) 5 μg/mL for 48 h were determined by Western blotting. The bands were quantified using a scan densitometer. The protein levels were normalized to the loading control GAPDH. Data are presented as mean±SEM of three independent experiments; ⁎ p <0.05 when compared to untreated cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Expressing, Western Blot, Control

Journal: Leukemia Research Reports

Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

doi: 10.1016/j.lrr.2016.08.001

Figure Lengend Snippet: Effect of MEM-M6/6 anti-CD147 on MDR1 (A) and BSG (B) mRNA levels in drug-resistant K562/ADR cell line. The quantification of mRNA expression in the presence of various concentrations of MEM-M6/6 (0–5 µg/mL) were normalized to the loading control GAPDH mRNA. The ratio of mRNA was calculated for each group. Data are presented as mean±SEM of three independent experiments; ⁎ p <0.05 when compared to untreated K562/ADR cell.

Article Snippet: This study used the following antibodies: mouse anti-CD147 monoclonal antibody clone MEM-M6/6 (Exbio, Vestec, Czech Republic); mouse anti-P-gp monoclonal antibody (MT PGP1) (Biomedical Technology Research Center, Chiang Mai, Thailand) [ ], mouse anti-γ 4 globin monoclonal antibody (PB1) (Biomedical Technology Research Center) [ ] , rabbit anti-CD147 polyclonal antibody (Abcam, Cambridge, UK) , rabbit anti-GAPDH polyclonal antibody (Merck Millipore, Billeric, MA, USA), goat anti-rabbit IgG-HRP (Abcam), and rabbit anti-mouse Igs-FITC (Dako, Hamburg, Germany).

Techniques: Expressing, Control